conditioned medium, mesenchymal cells, skin wound
healing, EGF,diabet
Investigating the Effects of Conditioned Medium of Human Wharton's Jelly Mesenchymal Stem Cells on EGF Gene Expression in Skin Wound Healing in Streptozotocin-Induced Diabetic Rats
Abstarct
Introduction & Objective: Conditioned medium of umbilical cord mesenchymal cells is an environment rich in various growth factors and cytokines, the use of which causes self-improvement and self-renewal in damaged tissues. In this study, we aimed to investigate the effect of conditioned medium of Wharton's cord mesenchymal cells on EGF gene expression and skin wound healing in diabetic rats
Materials and Methods: In this study, 20 rats were anesthetized using xylazine and ketamine. The surgical method was used to create a wound, which was created with
a surgical puncture of a skin wound in the back area of the neck. Rats were divided into 2 groups of 10. In the treated diabetic group, conditioned medium the amount of 1 ml was used intradermally and in the diabetic control group, the same amount of physiological serum was used. . Two weeks after the injection, the animals were first anesthetized with ether. Part of the tissue samples were transferred to 10% formalin solution for histological studies and part of the dermal tissue samples were immediately frozen and transferred to the Genetics Laboratory of the Biotechnology Research Center of Tabriz Azad University for molecular
tests
Results: In this study, it was shown that wound healing increased in the diabetic rat group with conditioned medium compared to the diabetic group. Also, in molecular studies, it was found that the expression of EGF gene in the treated samples increased significantly compared to the control group
Conclusion: The results showed that the use of conditioned medium of human umbilical cord Wharton jelly mesenchymal cells improves the wound healing process in terms of tissue and also increases the expression of EGF gene as a wound control gene
