mesenchymal cells, Medium conditioning, skin wound healing
The effect of conditioned medium of umbilical mesenchymal stem cells on skin wound healing in diabetic Rats
Abstarct
Introduction & Objective: Medium conditioning was a substance obtained in this study from culture of umbilical cord mesenchymal cells. This environment is rich in various rowth factors and cytokines that result in self-improvement and self-renewal in damaged tissue. In this study, we aimed to investigate the effect of medium conditioning of Wharton's jelly mesenchymal cells on skin wound healing in diabetic rats
Materials and Methods: In this study, 30 rats were anesthetized using xylazine and ketamine. The surgical method was used to create a wound, which was created with a surgical puncture of a skin wound in the back area of the neck. Rats were divided into 3 groups of 10. In the control group, physiological serum was used and the diabetic group was divided into two groups
In the first group, diabetic rats did not use any substance and in the second group, diabetic rats, medium conditioning was used. After the study, tissue samples were taken along with healthy tissue on days 1, 5, 9, 15 and 17 and transferred to 10% formalin solution. Tissue staining was performed with hematoxylin-eosin and trichromason and the amount of inflammatory cells, fibroblasts, collagen and tissue epithelium and the percentage of wound healing were measured
Results: In this study, it was shown that wound healing increased in diabetic rats with medium conditioning compared to the diabetic group and the rate of epithelium and fibroblast and inflammatory cell formation increased in diabetic rats with medium conditioning compared to diabetic
By examining the totality of the studies, it can be said that by using the medium condition of umbilical cord mesenchymal cells, the wounds caused by diabetes can be treated and the formation of epithelium, fibroblasts and inflammatory cells can be increased
