stem cell | Histochemical Study of Skin Following Injection of Conditioned Medium of Human Wharton's Jelly Mesenchymal Stem Cells in Skin Wound Healing in Streptozotocin-Induced Diabetic Rats
Abstract
Background and aim: The ability to repair injuries for living organisms is vital. The healing of the wound is a complex process involves interactions between different types of cells, growth factors and extracellular matrix compounds
Despite the advances in medical science and an increase in the understanding of the molecular processes of biological events, today diabetic wounds are still considered as a crisis for human health. One of the important issues we face is the lack of acceptable treatment for wounds that their treatment lasts. Today, we know well that
stem cells play an important role in tissue repair processes. Mesenchymal stem cells are known for their potential for different cells in different environments. Nevertheless, nutritional effects and their immune modulus on adjacent cells can be considered as a therapeutic role. The aim of this study was to evaluate the amount of collagen by western blot in skin tissue following the injection of medium concentration of mesenchymal cells in human skin ulcer
Performing this research is an interventional type and has been incorporated in the stages. In this experimental study, 20 adult male Wistar rats with streptozoline were used in a weight range of 250 ± 20 g. Diabetic rats were divided into two groups of 10. The first group as the control group did not intervene as the control group. The second group was placed in human cord stem cells after the treatment of the culture
medium (Medium). After healing, the sample was improved in the form of biopsy and was sent to the laboratory along with the ice mold to measure collagen by Western blot method. The results showed that the amount of collagen in the treatment group was significantly increased by stem cell count. Due to the role of collagen in the wound healing process, the medium channel of mesenchymal stem cells can be considered as a potential treatment for hard diabetic wounds, although there is still a need for further studies on this issue
